Histology : histologie AT college-de-france.fr

 

 

Introduction

 

The LPPA histology workshopstand at the interface between the research teams with which it is involved and the neuroimaging technical center. It carries out different classical and immunohistochemical stainings as well as revelation in simple or double labeling of neuronal or transneuronal tracers.

 

Front section through the hippocampus
Nissl staining: Cresyl violet
	S. Wiener
Visual cortex
Cresyl violet	Cytochrome oxidase
	C. Milleret
Immunohistochemistry: visual Cortex
Labeled Astrocytes: FITC fluorescence	Detail
Neuroimaging Photography	C. Milleret
Revelation in double labeling (biocytin, HRP)
Injection site of the pontine omnipause neurons of the saccade generator	HRP labeled reticulospinal neuron in contact with the Biocytin labeled synaptic boutons
Neuroimaging Photography	A. Grantyn
Immunological revelation of the transneuronal tracer (rabies virus)
	W. Graf

 

Research projects in which the technical center is involved

 

• Neuronal mechanisms implied in the behaviors of navigation and orientation.
  S. Wiener – laboratoire LPPA

• Seek of an asymmetry of visual interhemispheric integration via the corpus callousum in the event of strabism.
  C. Milleret – laboratoire LPPA

• Specificity of orientation of interhemispheric connections in the visual cortex: anatomical and functional study by optical imagery.
  C. Milleret – laboratoire LPPA

• Distribution of the astrocytes marked in GFAP, connexines 30 and 43 during the development.
  C. Milleret et C. Giaume – laboratoires LPPA et Neuropharmacologie

• Morphological study of the neurons implied in the ocular jerks .
  A. Grantyn – laboratoire LPPA

• Study of the neuronal circuits of the movement of the eyes.
  W. Graf et A. Grantyn – laboratoire LPPA

• Study of the subcortical and cortical ways projecting on the parietal cortex.
  W. Graf et G. Ugolini – collaboration laboratoire NBCM